Clustering Class I MHC Modulates Sensitivity of T Cell Recognition

Clustering class I MHC modulates sensitivity of T cell recognition.

T cell recognition of peptide-MHC is highly specific and is sensitive to very low levels of agonist peptide; however, it is unclear how this effect is achieved or regulated. In this study we show that clustering class I MHC molecules on the cell surface of B lymphoblasts enhances their recognition by mouse and human T cells. We increased clustering of MHC I molecules by two methods, cholesterol...

Prediction of Cytotoxic T Cell (MHC Class I) epitopes

Regulation of protein translation through mRNA structure influences MHC class I loading and T cell recognition.

Many viruses avoid immune surveillance during latent infection through reduction in the synthesis of virally encoded proteins. Although antigen presentation critically depends on the level of viral protein synthesis, the precise mechanism used to regulate the generation of antigenic peptide precursors remains elusive. Here, we demonstrate that a purine overloaded virally encoded mRNA lacking se...

Peptide-Independent Alloreactivity Class I Molecule: Implications for T Cell Recognition of an Engineered MHC

Structural features underlying T-cell receptor sensitivity to concealed MHC class I micropolymorphisms.

Polymorphic differences distinguishing MHC class I subtypes often permit the presentation of shared epitopes in conformationally identical formats but can affect T-cell repertoire selection, differentially impacting autoimmune susceptibilities and viral clearance in vivo. The molecular mechanisms underlying this effect are not well understood. We performed structural, thermodynamic, and functio...